Looking back almost 10 years later... this moment is still one of the most unique and special ones of our wonderful artist's expressions.
The mix of emotions on Alison's face as she thinks about her boobs for the first time, and the thoughts going through her head as she actually confirms what she is for the first time. It's relatable to some, hot to most, and mesmerizing to all.
It's almost like she was once a very strong, powerful male fighter, the best in the world maybe. But she was punished at some point and turned the soft petite toy she is here. Stripped of her manhood, she maintained some of her old arrogance to a former sparring partner she had won against many times. And this was the end of that arrogance. Put in her place, she goes to clean the mats like a good girl, realizing her new place in life.
Independent of the story, her expression in this pic makes me think of a scenario where a thin weak nerd comes into a gym seeking to bulk up and become an "alpha" only to fail so miserably he gets fairly quickly turned into a girl as the hyper-masculine magic of the gym drains his manhood away. This is the facial expression just after being freshly transformed: pure bewilderment.